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. 2024 Apr 19;11(24):2307238. doi: 10.1002/advs.202307238

Figure 6.

Figure 6

ABs inhibited ferroptosis in ischaemic flaps via the KEAP1‐Nrf2 axis. a) IF staining of KEAP1 and Nrf2 in FLAP area II on POD7 in the five groups. Scale bar: 10 µm. b) Quantified integrated intensity of KEAP1 (top) and nuclear Nrf2 (middle) and the MOC for KEAP1‐Nrf2 (bottom) in the dermal layer in the five groups (n = 6). c) IF staining of SLC7A11, GPX4 and 4‐HNE in FLAP area II on POD7 in the four groups. Scale bars: 10 µm (SLC7A11, GPX4) and 20 µm (4‐HNE). d) Quantification of the integrated intensity of SLC7A11, GPX4 and 4‐HNE in the dermal layer in the four groups (n = 6). e) KEAP1, nuclear Nrf2 and ferroptosis‐related protein levels in FLAP area II in the four groups on POD7. f) Comparison of the expression levels of KEAP1, nuclear Nrf2 and ferroptosis‐related proteins in the four groups. β‐Actin and Histone 3‐H3 served as loading controls for band density normalization (n = 6). g) Quantification of the LPO (left) and iron (right) levels in area II of the FLAP in the four groups (n = 6). h) ELISA analysis of area II of the FLAP to examine the COX2 (top) and NOX1 (bottom) levels in the four groups on POD7 (n = 6). i) MDA (top) and GSH (bottom) levels in area II of the flap in the four groups on POD7 (n = 6). j) ELISA analysis of TNF‐α (top) and 8‐OHdG (bottom) levels in area II of the flap in the four groups on POD7 (n = 6). k) ELISA of IFN‐β (top) and HMGB‐1 (bottom) levels in area II of the flap in the four groups on POD7 (n = 6). The error bars are the SEMs. Significance (*): p value < 0.05; ANOVA plus the LSD post hoc test (equal variances) or Dunnett's T3 method (unequal variances).