Figure 3.

Self‐healing properties of coiled‐coil protein‐based hydrogels. G′: storage modulus represented by dark‐colored closed circles, G″: loss modulus represented by light‐colored open circles. PXP in red, T40A in blue, Q54A in green, and T40A+Q54A in orange. a) Representative cyclic strain sweep test at 37 °C and 30 rad s⁻¹ with 5% low strain value and 500% high strain value (highlighted in gray). Full recovery is achieved for each gel type after each of 4 periods of high strain. b) Zoom of a representative high strain period from the cyclic strain sweep. Visually slower recovery of PXP is observed when compared to the mutants. c) Average recovery time crossover, when G′ > G″ after the high strain period, is improved for mutants by 3 s at 37 °C. d) Average storage modulus for each gel type (10% w/v) and temperature conditions with no statistical difference between mutants. Bars represent the mean of N = 5 replicates, error bars indicate SEM. A multiple comparisons two‐way ANOVA table was implemented for mutant comparison (* p < 0.05).