Figure 5.

Cell viability is maintained following injection in XTEN‐based coiled‐coil gels. a) Live/dead analysis of fibroblasts 24 h following injection in 70% ethanol, media, or PXP imaged using confocal microscopy following calcein/ethidium homodimer staining. These conditions are compared to a no injection control, to demonstrate viability prior to both gel encapsulation and injection. Live cells are shown in green, dead in red. b,c) Fibroblast viability quantified through NucleoCounter analysis 24 h‐ and 72‐h post injection in the mutant gel constructs. d) Confocal live/dead analysis of hESC‐CMs 1 day following injection in 70% ethanol, media, or PXP. e,f) NucleoCounter analysis of hESC‐CMs viability 24‐ and 72‐ h following injection in all mutant gels. Error bars reported as SEM, N = 3. Scale bar = 200 µm.