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One-step growth curves of parental and chimeric viruses in HeLa cells. (A) Replication of cardiovirulent CVB3/20, noncardiovirulent CVB3/CO, and intratypic chimeras; (B) replication of cardiovirulent CVB3/20, CVB3/AS, and intratypic chimeras. Cells were inoculated as described in Materials and Methods and harvested by freezing at specific times shown. Virus titers were determined on HeLa cell monolayers. p.i., postinoculation.
