TABLE 1.

Primers used for the amplification and sequencing of CVB3

Name	Sequence	Locationa	Orientationb
T1	5′-TCACTATAGGTTAAAACAGC-3′	1	S
JRp64	5′-ACGGTACCTTTGTGCGCCTGTTTTA-3′	63	S
JRp577	5′-TGGCTGCTTATGGTGACAAT-3′	582	S
B3-CO/Sac I	5′-GATGGGAGCTCAAGTGTCG-3′	742	S
JRpATG	5′-TGAACTCGAGGGTA-3′	756	A
B3-1226-CC	5′-GGGCAAAACATGCAATATCACTACTTRGG-3′	1226	S
B3-1464	5′-GTGTATAAYGCAGGCATGGG-3′	1464	S
3′PUFF	5′-ATGTTATCCATAGGTACACT-3′	1593	A
B3-1990	5′-GGCTTTCCACTGCAACCRGG-3′	1990	S
ID3	5′-CACGCCACGTTAAGAACTACC-3′	2601	S
B3-2689	5′-ACTCGGTGAAGTAAACACATGC-3′	2689	A
B3-2729	5′-ATCACCCATTCRGCATACCG-3′	2729	A
B3-AS/Spe I	5′-GTTATAACTAGTACTCAACAGCC-3′	2806	S
AS/20(3324)S	5′-GGCGCTTTTGGACAACAATCAGGGGCAG-3′	3296	S
B3-3324	5′-ACTGCCCCTGATTGTTGTCC-3′	3324	A
20/ApaL I	5′-CCCGTTGTGCACTGACATCTGG-3′	3477	A

^aNumbered from the 5′ terminus relative to the CVB3/20 genome (46). 

^bS, sense; A, antisense.