FIG. 5.

Viral gene expression after infection of MDMs. Each virus was prepared by cotransfection to COS cells with pNL43lucΔenv vector and pJD-1 (A, C, and D) or HIV-1 macrophage-tropic envelope expression vector (pJR-FL) (B). The supernatants harvested and treated with DNase at 48 h posttransfection were used to inoculate MDMs (A, B, and D) or RD cells (C). At 4 days postinfection, the entire cells were harvested and washed with PBS. Cell pellets were lysed with 150 μl of luciferase lysis buffer (Promega). Ten microliters of each cell lysate was subjected to the luciferase assay as described in Materials and Methods.