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. 2024 Jun 25;7(9):e202302250. doi: 10.26508/lsa.202302250

Figure 5. Nrg1 signaling enhances the elongation of callosal axons in vivo.

Figure 5.

(A) Schematic representation of developing callosal axons at P2 in control littermates and in neurons expressing Nrg1-FL. Arrows indicate the electroporated area, and the empty arrowheads show the location of the more advanced axonal tips (top axons). The images below show the electroporated area, the top axons, and their tracing. The arrowheads indicate the tip of the axons. Scale bar, 50 μm. (B, C) Quantification of axonal extension in Nrg1-FL–expressing neurons relative to control littermates. (B, C) Bar graph shows the mean ± SEM (B), whereas the plot shows the cumulative frequency (C). Ctrl: n = 60 axons, out of six sections from four brains; Nrg1-FL: n = 90 axons, out of nine sections from four brains. (B) Unpaired t test, *P < 0.05. (C) Kolmogórov–Smirnov test, *P < 0.05. (D) Drawing shows the callosal axons at P2 in control littermates and in Nrg1-ICD–expressing neurons. Arrows indicate the electroporated area, and the empty arrowheads show the location of the more advanced axonal tips (top axons). The pictures show representative images of the electroporated area, the top axons, and their tracing. The arrowheads indicate the tip of the axons. Scale bar, 50 μm. (E, F) Bar graph shows the mean ± SEM (E), whereas the plot shows the cumulative frequency (F). Ctrl: n = 90 axons, out of nine sections from three brains; Nrg1-ICD: n = 60 axons, out of six sections from three brains. Both conditions are from one litter, counting with internal controls within littermates. (E) Unpaired t test, **P < 0.01. F, Kolmogórov–Smirnov test, **P < 0.01.