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. 2024 Jun 8;13(6):704. doi: 10.3390/antiox13060704

Figure 1.

Figure 1

(A) Scheme of in vitro culture of embryos using a Transwell. Control: embryos incubated KSOM without Transwell; T-Control: no cells in the upper chamber; E-ASCs (adipose tissue-derived mesenchymal stem cells): early passage of ASCs (P3: 3rd passage) seeded in the upper chamber; L-ASCs: late passage of ASCs (P20: 20th passage) seeded in the upper chamber. (B) Multicolor flow cytometry analysis of cells in the plot of singlet cells based on staining for CD105, CD29, Sca-1, and CD45. The percentages of mesenchymal stem cells, including early passage (3rd passage) adipose tissue-derived stem cells (ASCs) and late passage (20th passage) ASCs, harboring the cell-surface expression markers CD29, Sca-1, and CD105, and negative CD45 are shown. Cells in pink box indicate mesenchymal stem cells. (C) Embryo morphology on Day 4. (D) Blastocyst and hatching rates of in vitro-produced mouse embryos co-cultured with ASCs. The mean percentage was calculated from three independent experiments. n = 150 per group. Data are presented as the mean ± standard error. * p < 0.05 vs. Control.