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. 2000 May;74(10):4816–4823. doi: 10.1128/jvi.74.10.4816-4823.2000

FIG. 1.

FIG. 1

Structure of HD-IFN and Ad-IFN and expression of mIFN-α2 in vitro. (A and B) mIFN-α2 expression cassette used in this study. The mIFN-α2 gene was cloned downstream of the TTR promoter (prom.) and enhancer (enh.) followed by the polyadenylation signal of bovine growth hormone (GH pA). Int., intron. (A) To generate the HD-IFN vector, the expression cassette was cloned in the SmiI site of the STK120 backbone vector, which contains the following sequence: the left-terminus Ad5 internal terminal repeats (ITR) and packaging signal (ψ); a fragment of the human hypoxanthine guanine phosphoribosyltransferase (HPRT); a human fragment of the C346 cosmid; and the right-terminus Ad5 ITR sequence. (B) To derive the Ad-IFN vector, the expression cassette was recombined in the E1 region of pHVAd1 vector as described in Materials and Methods. (C) The human cell lines Huh-7 and HeLa were transduced with either HD-IFN (open bars) or Ad-IFN (solid bars) at a multiplicity of infection of 10. Secretion of mIFN-α2 into the cell culture medium was measured by the VSV cythopathic inhibition assay as described in Materials and Methods.