Figure 3.

Effect of optimized SH extract on cell viability, ROS generation, and primary enzymes treated with and without H₂O₂ in RAW264.7 cells. Cells were pretreated with SH and gallic acid for 12 h, followed by treatment with or without 10 mM of H₂O₂ for 6 h. Cell viability with H₂O₂ (A). The generation of cellular ROS was evaluated using the DCFH-DA method (B); the protein expression of SOD1 and catalase was measured with Western blot analysis (C), and the relative protein expression was quantified using Image J software (Version 1.54i 03). Differences in the alphabetic letters represent statistical significance (p < 0.05) to one another.