Figure 4.

Activation of phase II antioxidant enzymes via the regulation of Nrf2. Optimized SH extract dose-dependently decreases the Keap1 and facilitates the translocation of Nrf2 protein expression into the nucleus (A), resulting in the upregulation of phase II enzyme expression (B) using immunoblotting assay. The relative protein expression was quantified using Image J software. Differences in the alphabetic letters represent statistical significance (p < 0.05) to one another. Gray: non treated; purple: sample treated; green: gallic acid treated.