Figure 3.
Effect of the antidepressants on ROS generation in Aβo-stimulated SH-SY5Y cells. (A,B) SH-SY5Y cells were treated with the three antidepressants + Aβo for 24 h after WAY-100635 pretreatment for 1 h. The p-value in ANOVA was <0.0001. Results are expressed as means ± SEMs. *, p < 0.0001, versus Aβo-exposed cells. #, p < 0.0001, versus Aβo + 0.1% DMSO-exposed cells. In the absence of 5 μM Aβo, ROS levels of Flx-, Dlx-, and Mir-treated cells were 29.6 ± 1.44 (p = 0.9927 vs. control), 27.7 ± 1.25 (p = 1.0000 vs. 0.1% DMSO control) and 23.9 ± 1.89 (p = 0.9997 vs. 0.1% DMSO control) fluorescence intensity × 106/μg protein (no significant difference, n = 10, Tukey’s test). (C–O) SH-SY5Y cells were observed using fluorescence microscopy. The scale bars represent 100 μm. Aβo, Aβ oligomer; DMSO, dimethyl sulfoxide; ROS, reactive oxygen species; Flx, fluoxetine; Dlx, duloxetine; Mir, mirtazapine; SEM, standard error of the mean; ANOVA, analysis of variance.