Figure 5.

Effect of the antidepressants on membrane phospholipid peroxidation in Aβo-stimulated SH-SY5Y cells. (A,B) SH-SY5Y cells were treated with the three antidepressants + Aβo for 30 min after WAY-100635 pretreatment for 10 min. The p-value in ANOVA was 0.0009. Each value expresses the mean ± SEM of 10 individually treated samples (Tukey’s test). *, p < 0.005, **, p < 0.001 versus Aβo and #, p < 0.05; ##, p < 0.005, versus Aβo + 0.1% DMSO-exposed cells. In the absence of 5 μM Aβo, membrane phospholipid peroxidation levels of Flx-, Dlx-, and Mir-treated cells were 1.85 ± 0.06, 1.90 ± 0.06, and 1.86 ± 0.07 fluorescence intensity × 10⁴/μg protein (no significant difference, n = 10, Tukey’s test). Aβo, Aβ oligomer; DMSO, dimethyl sulfoxide; DPPP, diphenyl-1-pyrenylphosphine; Flx, fluoxetine; Dlx, duloxetine; Mir, mirtazapine; NS, not significant; SEM, standard error of the mean; ANOVA, analysis of variance.