FIG. 5.

Localization of CPV capsids after microinjection into the cytoplasm of cells. (A) Differential interference contrast and fluorescence confocal image of cells 2 h after injection, showing the specificity of staining for the virus in the injected cells. (B) Full or empty CPV capsids injected into the cytoplasm of the cells that were either fixed immediately (0 h), or incubated for 1, 3, or 6 h at 37°C prior to fixation. The cells were stained for the capsid with MAb 8, followed by FITC-conjugated anti-mouse secondary antibody.