FIG. 6.

(A) Intracellular localization of capsids injected into cells in the presence of nocodazole. Full or empty capsids were injected into cells in the presence of nocodazole and then incubated for 1 h (1h ND) or for 6 h (6h ND). Other cells were injected in the presence of nocodazole, incubated for 6 h, washed, and incubated for a further 6 h in normal medium (6h ND + 6h chase). (B) Cells injected with full or empty capsids in the presence of nocodazole and then incubated for 12 h with the drug (12h ND). The cells were then fixed and stained with MAb 8 or with TxR-labeled anti-NS1 to distinguish those that were infected. (C) Cells that were untreated (−ND) or incubated for 1 h with 20 μM nocodazole (ND) were fixed and then stained with an antibody against tubulin.