FIG. 2.

Subcellular localization of wild-type Vpr and its mutant variants. Approximately 2 μg of plasmids which express Vpr and its mutants R73S and R73A, in fusion with GFP (EGFP), were introduced into T98G cells by the calcium phosphate precipitation method. After 36 h, cells were examined by fluorescence microscopy for the presence of the EGFP-Vpr proteins in the transfected cells. Cells were transfected with EGFP alone (panel A) or with wild-type Vpr EGFP, EGFP-R73S, or EGFP-R73A (panels B, D, and F, respectively). Panels C and E show cells transfected with EGFP-R73S and EGFP-R73A in the antisense orientations, respectively.