Skip to main content
. 2024 Jun 19;14(6):726. doi: 10.3390/biom14060726

Figure 4.

Figure 4

Necrosulfonamide (NSA) treatment reduces medial elastin breaks, smooth muscle cell (SMC) depletion, and collagen deposition in experimental abdominal aortic aneurysms. Apolipoprotein E-deficient mice were sacrificed 28 days following angiotensin II infusion. Aortas were harvested, sectioned (8 μm), and stained via the elastic Verhoeff’s Van Gieson staining for medial elastin, SMC α-actin antibody for SMCs, and Masson trichome staining for collagen deposition. (A) Representative histological staining images for elastin (black to blue/black), SMCs (red), and collagens (blue). (B,C) Quantification of medial elastin breaks. (D,E) Quantification of SMC α-actin-positive area in aortic cross-sections (ACSs). (F–H) Quantification of collagen-positive area in ACSs. All data in (C,E,G,H) are normalized by aortic internal perimeter in ACSs. Student’s t test ((D,F): mean ± standard deviation) and non-parametric Mann–Whitney ((B,C,G,H): median and interquartile range (25% and 75%)), * p <0.05, ** p < 0.01 and *** p < 0.001 compared to vehicle treatment.