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. 2024 Jun 8;12(6):1274. doi: 10.3390/biomedicines12061274

Figure 8.

Figure 8

Schematic summary of the layer-specific Sp5 regulation along the Hydra body axis. (A) Dot plot view of Zic4, Wnt3, Sp5, TCF, Wnt5A, Wnt8 and β-catenin expression in cells from the epithelial lineages, either epidermal or gastrodermal, and the interstitial lineage as deduced from Hydra single-cell transcriptome analysis [35]. Along the central body column, single-cell sequencing has identified two populations of epithelial stem cells in the epidermis (SC1 and SC2) and three in the gastrodermis (SC1, SC2 and SC3). See other abbreviations in Figure S1. (B,C) Schematic representation of the predicted genetic regulation network (GRN) at work in the epidermal and gastrodermal layers of the hypostome (B) and tentacle (C) regions in Hydra. (D,E) Schematic view of GFP fluorescence (green), Sp5 expression and predicted GRNs at work in the epidermal (D) and gastrodermal (E) layers of the body column (BC) of HySp5-3169:GFP transgenic animals, either maintained in homeostatic conditions (left) or ALP-treated, or knocked-down for Sp5 or β-catenin (right). Bold letters, black arrows and thick red bars indicate a stronger activity.