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. 2024 Jun 20;12(6):1373. doi: 10.3390/biomedicines12061373

Figure 5.

Figure 5

Matrix metalloproteinase zymography assessing MMP activity post-ICH and miR-195-5p treatment. The impact of ICH on the activity of various MMPs and the modulatory effects of miR-195-5p treatment. (A) The zymogram reveals the activity levels of Pro-MMP-9, MMP-9, and MMP-2, with samples from both unaffected (contralateral) and affected (ipsilateral) brain hemispheres across the control, ICH, and ICH-treated groups with either NC-mimic or miR-195-5p. It highlights a significant upregulation of Pro-MMP-9 and MMP-9 activities in the ipsilateral hemisphere following ICH, which is notably diminished upon treatment with miR-195-5p, indicating its therapeutic potential in mitigating MMP-related pathologies post-ICH. (B) The bar graphs present a quantitative comparison of these enzymes’ activities, showing relative enzyme densities to the control and demonstrating the specific reduction in MMP activity (particularly for Pro-MMP-9 and MMP-9) associated with miR-195-5p treatment. (*** p < 0.001, ** p < 0.01, and * p < 0.05 compared with the control group, ## p < 0.01 compared with the ICH + NC-mimic group) (n = 4 in each group).