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. 2024 Jun 15;13(12):1041. doi: 10.3390/cells13121041

Figure 3.

Figure 3

Lysosomal damage-induced cell death is specific to CLL cells. CLL cells were treated with 1 µM siramesine, 0.05 nM venetoclax alone, and in combination for 24 h. The cells were stained, and fluorescence was measured using flow cytometry. (A) The graph represents the loss of Lysotracker Red DND-99 with each treatment condition (N = 4 independent patient samples). (B) Cell death was determined by trypan blue exclusion assay following treatment. (C) AnnexinV-FITC/7-AAD was performed to analyze the total apoptotic cell death of CLL with each treatment condition. The CD3+ was stained prior to AnnexinV/7-AAD treatment and gated for the CD3+ T-cell marker. Error bars represent SEM. * represents statistical significance p < 0.05, ** represents statistical significance p < 0.01, *** represents statistical significance p < 0.001, and **** represents statistical significance p < 0.0001.