Table 1.
Quantifiler™ THP DNA Standard stock diluted in Quantifiler™ THP DNA Dilution Buffer (THP) was used for the construction of the standard curve. A minimum input volume of 10 μL DNA for dilutions was used to ensure the accuracy of manual pipetting, as suggested by the Quantifiler™ Manual. The concentration ranged from 50 ng/μL (St. 1) to 0.005 ng/μL (St. 5).
| Standard | Concentration (ng/µL) | Volume | Dilution Factor |
|---|---|---|---|
| St. 1 | 50.000 | 10 µL [100 ng/µL stock] + 10 µL QuantiFiler™ THP DNA dilution buffer |
2X |
| St. 2 | 5.000 | 10 µL [Std. 1] + 90 µL QuantiFiler™ THP DNA dilution buffer |
10X |
| St. 3 | 0.500 | 10 µL [Std. 2] + 90 µL QuantiFiler™ THP DNA dilution buffer |
10X |
| St. 4 | 0.050 | 10 µL [Std. 3] + 90 µL QuantiFiler™ THP DNA dilution buffer |
10X |
| St. 5 | 0.005 | 10 µL [Std. 4] + 90 µL QuantiFiler™ THP DNA dilution buffer |
10X |