Table 2.
Experiment with DNA controls dosed like samples. Each dilution of the control DNA mixture used to construct the curve was assayed five-fold as if it were an unknown sample. The average values of the measurements, the standard deviation, and the percentage difference with respect to the respective theoretical points were calculated. The small human autosomal probe (Small) consists of diploid multi-copies of 80 bp, the large human autosomal probe (Large) of diploid multi-copies of 214 bp, the human male target (Y) of haploid multi-copies of 75 bp, and the synthetic IPC probe of 130 bp. The whole data of the experiment are available in Table S1.
| Theoretical Input in ng/µL | ||||||
|---|---|---|---|---|---|---|
| 50 | 5 | 0.5 | 0.05 | 0.005 | ||
| probe | ||||||
| Large autosomal | average | 50.504 | 5.435 | 0.510 | 0.053 | 0.005 |
| standard deviation | 5.503 | 0.227 | 0.028 | 0.003 | 0.001 | |
| % difference (BIAS) | 1.008 | 8.692 | 2.077 | 5.188 | 6.312 | |
| Small autosomal | average | 41.664 | 4.963 | 0.498 | 0.055 | 0.006 |
| standard deviation | 13.068 | 0.318 | 0.090 | 0.003 | 0.001 | |
| % difference (BIAS) | −16.672 | −0.730 | −0.309 | 10.492 | 15.734 | |
| Y | average | 41.237 | 4.934 | 0.474 | 0.057 | 0.005 |
| standard deviation | 7.154 | 0.517 | 0.062 | 0.009 | 0.001 | |
| % difference (BIAS) | −17.526 | −1.321 | −5.145 | 13.536 | 1.384 | |