Figure 6.

Neuroprotective effects of carotenoids against the neurotoxicity induced by AβO and 6-OHDA in SH-SY5Y cells. (A,B) Cells were treated at the same time with the studied carotenoids (5 µM) and AβO (10 µM) or 6-OHDA (100 µM) for 4 h and 2 h, respectively; (C,D) cells were treated with the studied carotenoids (5 µM) for 24 h and then with AβO (10 µM) or 6-OHDA (100 µM) for 4 h and 2 h, respectively. At the end of the treatments, the neuronal viability was determined using MTT assay. Data are expressed as percentages and shown as mean ± SD of three independent experiments (§§§ p < 0.001 and §§ p < 0.01 vs. untreated cells; * p < 0.05 and *** p < 0.001 vs. cells treated with AβO or 6-OHDA, one-way ANOVA with Tukey post hoc test).