FIG. 5.

Effects on the kinetics of accumulation of PrPres in spleen of a 2-h pretreatment with either MS-8209, DS500, or Congo red. Mice were treated with 25 mg/kg of either MS-8209 (◊), DS500 (▵), or Congo red (○) 2 h before intraperitoneal scrapie infection as described in Materials and Methods. Control mice (■) were treated with 5% glucose. PrPres purification and quantification were performed on spleen tissues harvested at 4 h and 7 and 22 days after infection. The standard deviations for results without error bars were too small to be represented. ∗, the difference between treated and control mice was statistically significant (P < 0.05; Mann-Whitney u test). A representative immunoblot at day 7 is shown.