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. 2024 Jun 15;46(6):6085–6099. doi: 10.3390/cimb46060363

Figure 1.

Figure 1

Schematic of wild-type tethered eel LH (LH-wt) and mutant tethered eel LH (LH-M) inserted eLH/CG β-subunit CTP region. The tethered form of eel LH β/α-wt was engineered to contain the β-subunit and common α-subunit sequences. The eLH/CG β-subunit carboxyl-terminal peptide linker was inserted between the β-subunit and α-subunit in the eel LH-M mutant using a polymerase chain reaction. The eLH/CG β-subunit CTP linker has 35 amino acids (115–149aa) and approximately 12 O-linked oligosaccharide sites. The numbers indicate the amino acids of the mature protein, except for the signal sequences. The encircled “N” denotes N-linked glycosylation sites at the eel LH β- and LH α-subunits, respectively. Green, FSH β; blue, α-subunit; and skyblue, eLH/CG β-subunit CTP linker. eLH/CG β CTP (115–149) represents the amino acid sequences of the eLH/CG β-subunit CTP linker. Red in the eLH/CG β CTP (115–149) denotes potential O-linked oligosaccharide sites.