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Quantity of rec-eel LH-wt secreted from CHO DG44 cells based on the day of culture. Supernatant was collected on days 0, 1, 3, 5, 9, and 11 of culture. The expression levels of the tethered rec-eel LH-wt protein were analyzed using a sandwich enzyme-linked immunosorbent assay, as described in the Methods section. Data for five clones are presented as representatives. Values are expressed as mean ± standard error of mean of at least three independent experiments.
