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Deglycosylation of the tethered eel LH-wt and LH-M proteins. The proteins from tethered eel LH-wt, LH-M 3, and LH-M 9 were treated with peptide-N-glycanase F to remove N-linked oligosaccharides, and western blot analysis was performed. The molecular weights of the tethered rec-eel LH-wt and LH-M proteins decreased by approximately 8–10 kDa.
