Table 1.
Bioactivity of rec-eel LH proteins in cells expressing eel LH receptor.
| Rec-LH Hormones | cAMP Responses | ||
|---|---|---|---|
| Basal a (nM/104 Cells) |
Log (EC50) (ng/mL) |
Rmax b (nM/104 Cells) |
|
| LH-wt | 0.9 ± 0.5 * | 138.8 * (116.6 to 171.4) c |
49.0 ± 1.2 * |
| LH-M | 2.1 ± 0.8 * | 47.8 ** (40.1 to 57.4) |
63.4 ± 1.5 ** |
Values are the means ± SEM of triplicate experiments. Log (EC50) values were determined from the concentration-response curves from in vitro bioassays. a Basal cAMP level average without agonist treatment. b Rmax average cAMP level/104 cells. c Geometric mean (95% confidence intervals) of at least three experiments. *, ** Values with different superscripts are significantly different (p < 0.05).