Table 1.
Antibodies used for immunoblotting. Antibodies against CFTR are raised to target different domains of the complex CFTR protein: ab660 (epitope NBD1, aa 405–436), ab217 (epitope RD, aa 807–819), ab570 (epitope RD, aa 731–742), and ab596 (epitope NBD2, aa 1204–1211).
| Antibody | Host/Clonality | Dilution | Provider | Cat. No. |
|---|---|---|---|---|
| CFTR | Mouse/ Monoclonal |
1:400 | CF Foundation (Bethesda, MD, USA) |
217, 660, 570, 596 |
| Vinculin | Mouse/ Monoclonal |
1:500 | Abcam (Cambridge, UK) | ab130007 |
| IgG H&L (HRP) | Goat anti-mouse | 1:7500 | Abcam (Cambridge, UK) | ab97040, Abcam |