Table 2.
CFTR cDNA expression in immune cell lines and primary immune cells was assessed by qPCR and referenced against the expression level of epithelial T84 cells.
| Probe ** | Amount (ng) |
CT Value CFTR |
Calibration on T84 (Based on Amount) | δCT (Probe—CALIBRATION) * | Fold Difference T84 # |
|---|---|---|---|---|---|
| 16HBE | 50 | 20.2 | |||
| T84 2 | 50 | 20.6 | 0.0 | 1 | |
| THP-1 undifferentiated 1 | 50 | Undetermined | 20.6 | CFTR mRNA beyond lower limit of detection | |
| THP-1 differentiated 1,2,3,S1 | 50 | 34.4 | 20.6 | 13.8 | 670 |
| Jurkat T cells **,S1 | 50 | 30.6 | 20.7 | 9.9 | 105 |
| CD14+ Monocytes 1 | 50 | Undetermined | 0.0 | CFTR mRNA beyond lower limit of detection | |
| CD19+ B cells 1 | 50 | Undetermined | 20.2 | CFTR mRNA beyond lower limit of detection | |
| CD8+ T cells 1 | 50 | Undetermined | 20.2 | CFTR mRNA beyond lower limit of detection | |
| CD16+ NK cells 1 | 50 | 36.5 | 20.2 | 16.2 | 2067 |
| CD4+ T cells unstimulated 1,2,S1 | 50 | 35.2 | 20.2 | 15.0 | 934 |
| CD4+ T cells stimulated S3,3 | 30 | 34.9 | 21.4 | 13.5 | 585 |
** RNA preparation was carried out independently twice. qPCR analyses were run in duplicate. Table displays mean values of all analyses per biomaterial source. * δCT = CT(T84) − CT(Immune cell); # Fold difference: 1.6 δCT, 1 Western blot depicted in Figure 1. 2 Protein detection depicted in Figure 2. 3 Protein detection depicted in Figure 3. S1 Protein detection depicted in Supplementary Figure S1. S3 Protein detection depicted in Supplementary Figure S3.