Figure 3.

Microinjection of dsRNA for RNAi in Ae. Aegypti: (a) RNAi significantly reduced the relative expression of Ae-GRD mRNA. (b) After feeding on a mixture containing 15 mg/mL ivermectin and 8% sucrose solution for 24 h, the mortality rate in the dsRNA-Ae-GRD group was 40.37%, which was lower than those observed in the blank and negative control groups. (c) GABA content in Ae. aegypti was significantly increased following RNAi treatment. Data represent three biological replicates, each consisting of three technical replicates. Statistical analysis was conducted using one-way ANOVA. “ns” indicates no significant difference, while asterisks denote levels of statistical significance: * p < 0.05, ** p < 0.01, **** p < 0.0001.