Figure 5.

Microinjection of miR-71-5p mimics: (a) Injection of miR-71-5p mimics significantly increased the expression of miR-71-5p in Ae. aegypti compared with the control group. (b) Overexpression of miR-71-5p led to a substantial downregulation of Ae-GRD expression. (c) Mortality was significantly reduced in the miR-71-5p mimics group after 24 h of treatment with ivermectin. Data represent three biological replicates, each consisting of three technical replicates. Statistical analysis was conducted using the t-test. Asterisks denote levels of statistical significance: ** p < 0.01, *** p < 0.001.