FIG. 2.

Intracellular expression and accumulation of mutated forms of δAg-S and δAg-L. Huh7 cells were cotransfected with combinations of two plasmids that expressed different forms of δAg. Four days later, the proteins were assayed by electrophoresis and immunoblotting to detect δAg species. In each cotransfection, as an internal standard, we used a third plasmid which expressed δAg-S(Δ19–31). Relative to this standard, the cells received threefold-greater amounts of the two plasmids that express forms of δAg-S and δAg-L. As indicated for lanes 1 to 10, these two forms were either wild type (wt) or one of the nine mutants.