TABLE 4.

Effect of mutations on ability to form complexes with δAg-S(FLAG)

Mutation in δAg speciesa	Pairing of δAg-S and δAg-L with δAg-S(FLAG)b
	δAg-S mutant/δAg-S(FLAG)			δAg-L mutant/δAg-S(FLAG)
	T	B	B/T	T	B	B/T
R13	5.9	2.2	0.37	0.31	0.29	0.96
L17	0.91	0.88	0.97	0.39	0.47	1.2
W20	1.2	0.33	0.27	0.36	0.13	0.36
V21	1.6	1.2	0.76	0.83	1.4	1.7
R24	2.4	1.5	0.65	0.81	0.98	1.2
W50	1.4	0.27	0.19	0.48	0.11	0.22
L51	1.6	0.69	0.43	0.46	0.28	0.61
I54	2.2	0.56	0.25	0.52	0.28	0.53
I57	2.3	0.67	0.29	0.73	0.23	0.31

^aAs described in Fig. 1. 

^bThe cotransfections used in these immunoaffinity experiments involved plasmids expressing the mutant forms of δAg-S or δAg-L, along with expression of δAg-S(FLAG) and δAg-S(Δ19–31). Other details are described in Materials and Methods. The immunoblot data shown in Fig. 6 were quantitated and used to deduce the values indicated for the ratio of δAg-S mutant or δAg-L mutant to δAg-S(FLAG) in the intact transfected cells (T) or in the fraction immunoaffinity purified from these cells (B). Also shown is the ratio B/T. As positive controls, the immunoaffinity data for wild-type δAg-S or δAg-L to δAg-S(FLAG) gave values for T, B, and B/T of 9.9, 3.6, and 0.37 and of 17, 7.3, and 0.42, respectively.