Figure 3.

ITC calorimetric studies. (A) A representative titration calorimetry curve of unilamellar vesicles composed of SM/Chol/GT1b ganglioside (47.5/47.5/5 mol ratio) with Aβ42 peptide fibrils. The calorimetric trace was observed upon successive injections of lipid vesicles into an Aβ42 solution contained in the reaction cell. (B) Cumulative heats of reaction obtained from the integration of the peaks are displayed in the top plot. The solid line represents the fitting of the experimental data to a partitioning model. The calorimetric cell was filled with a 28 μM Aβ42 solution. Lipid vesicles at a 35 mM lipid concentration were injected into the cell (1.43 mL) in 10-μL steps, leading to a 143-fold dilution of lipid vesicles. The titration experiments were performed at 37 °C. (Reprinted with permission from [11], 2021, Elsevier).