FIG. 2.

Analysis of reovirus particle density shifts in the presence of lysate containing μNS. Reovirus cores (A), virions (B), and ISVPs (C) (2 × 10¹²) were incubated with 50 μl of buffer or insect cell lysate containing μNS. Lysate was also incubated alone. Samples were subjected to equilibrium centrifugation in CsCl density gradients and fractionated. The buoyant density and OD₂₈₀ were determined for each fraction. The strong absorbance seen at the top of the gradient (lowest density) is due to detergent in the lysis buffer.