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. 2000 Jun;74(12):5516–5524. doi: 10.1128/jvi.74.12.5516-5524.2000

FIG. 7.

FIG. 7

Incubation of cores with μNS lysate and/or μ1 and ς3 lysate. Cores were incubated alone or with combinations of lysate containing μNS or μ1 and ς3. Samples were layered on CsCl density gradients and spun to equilibrium. Gradients were fractionated, and the fractions were subjected to immunoblot analysis. (A) Cores incubated with lysis buffer. (B) Cores incubated with μ1 and ς3 lysate. (C) Cores incubated with μNS lysate. (D) Cores incubated with μ1 and ς3 lysate followed by addition of μNS lysate and further incubation. (E) Cores incubated with μNS lysate followed by addition of μ1 and ς3 lysate and further incubation. (F) μ1 and ς3 lysate and μNS lysate mixed, followed by addition of cores and incubation. Antibodies used in the immunoblots are listed to the left, and proteins are labeled on the right. The top and bottom of the gradients are labeled below. The positions of recoated cores and cores are labeled at the top.