Figure 6.
The flow of cytoplasmic chloride (F(CS)%) evoked by muscimol application was assessed for the control (n = 9), CPSP (n = 11), and P2X7/CPSP (n = 8) groups. (A) The process of MEQ infusion into the brain slice and the rationale for assessing chloride amounts. (B) Example images of MEQ fluorescence expression of the control, CPSP, and P2X7/CPSP groups. The strength of MEQ fluorescence gradually declined by light shooting. The chloride ion channel was opened by muscimol induction, and the immediate [Cl−]in change was shown by MEQ fluorescence measurement. (C) The F(CS)% of the control, CPSP, and P2X7/CPSP groups was assessed after muscimol application over 25 min. P2X7/CPSP: P2X7 receptor knock-out mice that induced the CPSP symptoms.