Figure 8.

Mechanical pain and thermal pain were tested after thalamic hemorrhagic lesion induction. (A) The experiment was conducted. First, all mice were tested for mechanical pain via the von Frey test and thermal pain via the plantar test at baseline before inducing the VBC lesion of the thalamus. The VBC nuclei were destroyed on Day 0. The behavioral tests (i.e., the von Frey and plantar tests) were performed on Days 7, 14, 21, 28, and 35. After the behavioral tests, electrophysiological recordings, MEQ staining, ELISA, Western blotting, and immunostaining were performed. (B) Examples of collagenase lesions in the VBC. (C) Histological images of the VBC lesion. VBC: ventrobasal complex nuclei (i.e., the ventral posteromedial [VPM] and ventral posterolateral [VPL] portions).