TABLE 1.

Antiviral action of interferon against EMCV in the UHCV cell line depends on expression of the HCV polyprotein^a

MOI	Polyprotein expressed	EMCV yield (log PFU/ml)		ΔLog PFU/ml	Virus rescueb
		CONT	IFN
0.1	No	8.27 ± 0.39	4.47 ± 0.38	3.79 ± 0.49
	Yes	7.61 ± 0.41	4.89 ± 0.50	2.73 ± 0.31	11.68c
0.01	No	8.33 ± 0.33	3.31 ± 0.27	5.02 ± 0.53
	Yes	7.60 ± 0.33	4.54 ± 0.43	3.06 ± 0.23	90.40d

^aUHCV-11 cells were infected at MOI of 0.01 or 0.1 PFU/cell in the absence (control [CONT]) or presence of IFN at 500 U/ml and under conditions by which expression of the HCV polyprotein was repressed or induced. After 18 h of infection, the virus yields (expressed as mean log PFU/ml ± standard deviation [n = 6]) were titrated on Vero VC10 cells as described in Materials and Methods. The antiviral effect of IFN is given by the difference (Δlog) between the virus yields of CONT and IFN-treated cells. 

^bVirus rescue indicates the ability of the cells induced for the expression of the polyprotein to develop resistance to the antiviral action of IFN. This rescue was calculated as 10^ΔΔ, where ΔΔ represents the difference between the Δlog values of cells repressed or induced for the expression of the polyprotein. The reproducibility of the data was analyzed statistically by one-way analysis of variance (Duncan's multirange test), using the computer program Statview 4.5. 

^cP = 0.02. 

^dP = 0.003.