FIG. 6-7.

Latent gene expression using second-generation promoters. X-Gal staining of DRGs was after footpad inoculation with ICP34.5- and vmw65-deficient viruses 1764/pR19CMVlacZ, 1764/pR19minNSElacZ, and 1764/pR19MMLVlacZ. Expression from each of the viruses 1 month after inoculation is shown. (Top) pR20.5 and pR20.9 expression cassettes inserted into vectors deficient in ICP34.5 and vmw65. (Bottom) Gene expression characteristics at 3 days and 2 months after footpad inoculation with the pR20.9-containing virus (1764/pR20.9/43). DRGs were either stained with X-Gal for lacZ expression or viewed by fluorescence microscopy for GFP expression. RSV, Rous sarcoma virus.