Table 1.
Anthraquinone aglycones in Cortinarius sanguineus extract fraction 1. Sample’s concentration at 1 mg/mL in MeOH, MS m/z 50–750. sh = shoulder. The numbering of the peaks refers to the HPLC chromatogram shown in Figure 1. The calculated peak areas for compounds 1–3 do not correspond directly to abundance of the compound in the extract. References: [15,24].
| No. | Rt (min) | λmax (nm) | A430/A254 | A430/A285 | MS(−) m/z (%) | MS/MS(−) m/z (%) | MS(+) m/z (%) | Molecular Formula (Mass) | Proposed Compound | Peak Area % * |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 33.8 | 224, 284, 432 | 0.81 | 0.41 | 621 (65) [2M − 2H + Na]−, 299 (100) [M − H]− | 299: 284 (100) [M − H − CH3]− | 339 (11) [M + K]+, 323 (33) [M + Na]+, 302 (100) [M + 2H]+ | C16H12O6 (300.26) | Dermoglaucin | >5 |
| 621: 299 (100) [M − H]−, 284 (41) [M − H − CH3]−, 256 (13) [M − H − CH3 − CO]− | ||||||||||
| 2 | 39.6 | 228, 254, 268, 288, 440 | 0.61 | 0.54 | 269 (100) [M − H]− | 269: 225 (100) [M − H − CO2]− | 271 (100) [M + H]+ | C15H10O5 (270.24) | Emodin | >10 |
| 3 | 41.2 | 226, 263, 276sh, 306sh, 462sh, 485, 514sh | 0.35 | 0.46 | 653 (18) [2M − 2H + Na]−, 315 (100) [M − H]− | 315: 300 (100) [M − H − CH3]− | 355 (13) [M + K]+, 339 (27) [M + Na]+, 318 (100) [M + 2H]+ | C16H12O7 (316.26) | Dermocybin | >10 |
| 653: 315 (100) [M − H]−, 300 (33) [M − H − CH3]−, 272 (6) [M − H − CH3 − CO]− |
* Calculated from the 285 nm chromatogram.