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Representative NERT assay of wild-type and TAR mutant viruses. (A) negative strong-stop DNA detected by PCR in NERT reactions with TAR1, TAR2, TAR3, TAR4, TAR5, TAR5, TAR6, or wild-type virus (lanes 1 to 7, respectively) or mock supernatant (lane 8). (B) PhosphorImager analysis of the PCR shown in panel A; DNA copy number is indicated. (C) PCR standard curve generated using an HIV-1 proviral plasmid. (D) PhosphorImager analysis of the gel shown in panel C; r2 = 0.996.
