TABLE 1.
Analysis of partially purified virusa
Virusb | p24 Ag (ng/ml)c | RT activity (nU/μl)d | p24 Ag/RTe | Virus replicationf (%) |
---|---|---|---|---|
TAR1 | 115 | 3.0 | 38.3 | Negative |
TAR2 | 92 | 2.1 | 43.8 | Negative |
TAR3 | 103 | 2.4 | 42.9 | Negative |
TAR4 | 257 | 7.8 | 32.9 | 3 |
TAR5 | 1,212 | 26.9 | 45.0 | 5 |
TAR6 | 261 | 8.2 | 31.7 | 100 |
Wild type | 714 | 15.8 | 45.2 | 100 |
The results are representative of those from three independent experiments.
The TAR RNA sequence is shown in Fig. 1.
Concentration of p24 Ag measured in virus pelleted through a 20% sucrose cushion.
RT activity of virus pelleted through a 20% sucrose cushion.
Absolute value of p24 Ag concentration divided by RT activity.
Peak p24 Ag concentration in culture supernatant relative to the wild-type level.