Figure 5.

Immunomodulatory capacities of wild-type Lacticaseibacillus rhamnosus CRL1505 and L. rhamnosus Δmbf CRL1505 strain on the respiratory innate antiviral immune response triggered by TLR3 activation. Alveolar macrophages isolated from the wild type and the Δmbf CRL1505 mutant were challenged in vitro with the TLR3 agonist poly(I:C). IFN-β, IFN-γ, Il-12, IL-6, IL-10, and IL-27 levels were analyzed by ELISA after 24 h of TLR3 activation. Non-lactobacilli-treated alveolar macrophages challenged with poly(I:C) were used as controls. Data from three independent experiments are shown. Different letters indicate statistically significant differences (p < 0.05).