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. 2020 Dec 18;6(51):eabd7272. doi: 10.1126/sciadv.abd7272

Fig. 1. Overnutrition leads to β cell loss in zMIR fish.

Fig. 1

(A) Schematic of multiple sessions of overnutrition in zebrafish. Each session consisted of an 8-hour overnutrition treatment as indicated by the yellow rectangle and a 16-hour treatment in nutrient-free media as indicated by the white rectangle. Time 0 is the beginning of the first overnutrition session. (B) β cell number in fish challenged with multiple sessions of overnutrition. Data represent means ± SEM (n > 20 fish per time point). ***P < 0.001; two-way ANOVA, Tukey’s multiple comparisons test. (C to D′) Images of Tg(ins:H2BmCherry)-labeled β cells in control (C and D) and zMIR larvae (C′ and D′) at hours 56 and 72, respectively. Scale bars, 10 μm. (E) Whole-body free glucose content at hour 72. Data represent means ± SEM (n = 20 fish per group). *P < 0.05; one-way ANOVA, Tukey’s multiple comparisons test. (F) Insulin mRNA expression levels assessed by qRT-PCR in fish at hours 56 and 72. Data represent means ± SEM (n > 4 fish per group). *P < 0.05; one-way ANOVA, Tukey’s multiple comparisons test. ns, not significant. (G) Representative EM images of β cells at hour 64. Scales bar, 1 μm. (H) Quantification of granules per β cell section in islets from control and zMIR fish at hour 64. Data represent means ± SEM, n = 3 independent experiments. ***P < 0.001. (I) β cell number dynamics during the 16 hours of nutrient-free media from hours 56 to 72. Data represent means ± SEM (n > 25 fish per time point). ***P < 0.001; two-way ANOVA, Tukey’s multiple comparisons test. (J) Images of Tg(gcga:EGFP)-labeled α cells in the control and zMIR larvae at hour 72. (K) α cell number at hour 72 (n > 20 fish per group).