FIG. 1.
IL-10 inhibits detection of COX-2 mRNA (a and b), while IL-2 stimulates detection of COX-2 mRNA (b). PBMCs from PL animals and AL animals were cultured for 3 days with or without IL-10 and IL-2, and QC-PCR was performed. PCR products were separated on a 5% PAGE gel and stained with ethidium bromide. Quantification of gels was accomplished using Collagea software. Densitometric values for the standard curve were plotted as follows: log10([native RNA]/[competitor RNA]) versus log10 [native RNA (aM)]. The amounts of COX-2 mRNA were calculated based on the standard curve. The data is representative of three different PL animals tested (P2 [a] and P49 [b]) and three AL animals tested (S17 [a]). Standard errors of means are shown from at least three experiments on cells from the same animal.