FIG. 2.

PGE₂ increases detection of BLV tax and pol mRNA. BLV-infected AL and PL PBMCs were cultured for 3 days with IL-10 (10 ng/ml) and increasing concentrations of PGE₂ (0.1 and 1 μM) (a). BLV-infected PBMCs were also incubated with PGE₂ (1 and 10 μM) alone (b) and/or NS-398 (20 μM) (c). QC-PCR was performed as described in the text. The bands were analyzed using densitometry in the NIH Image program, version 1.61, and representative data are from one of three (a and b) or two (c) different AL and PL animals. Standard errors of means are shown from at least three experiments on cells from the same animal.