FIG. 3.

BLV proteins stimulate COX-2 mRNA (a) and BLV tax and pol mRNA (b). BLV proteins were purified from BL3* supernatant, and BLV-negative reagent was prepared from BL3 supernatant using metrizamide density gradient centrifugation. BLV-infected PBMCs were cultured for 3 days with similar volumes of purified BLV (10 μg/ml) and BLV-negative (BLV−) materials. COX-2 and BLV tax and pol mRNA were quantified as described above. Shown are representative data (PL animals) of experiments with three different AL and PL animals. Standard errors of means are shown from at least three experiments on cells from the same animal.