Figure 2.

Effect of SSD on LC3B and p62-mediated autophagy in CRC cells. (A) LC3 detection in CT26 and HCT116 cells treated with SSD for 48 h using flow cytometry. (B,C) Autophagy induction ratio in CT26 (B) and HCT116 (C) cells. (D,E) mRNA expression of LC3B and p62 was measured using real-time RT-PCR. (F,G) Protein levels of autophagic factors were detected using Western blot analysis. The results are presented as mean ± standard deviation of three repeated experiments, and the representative images are presented. *p < 0.05, ** p < 0.01, and *** p < 0.001.